Article info 2018 6 (3)    006  (03)   pp.  75 ~ 77
Title Expression, purification and crystallization of GSK3β in complex with the flavonoid morin
Authors Jeong Seok Cha1, Kuglae Kim1,2, Jinsook Ahn3 and Hyun-Soo Cho1*
Institutions 1Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea, 2Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27516, USA, 3Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea *Correspondence: hscho8@gmail.com
Abstract GSK3β is an important kinase that functions in cellular signaling pathways. Morin, a flavonoid that is plentiful in nature, was found as an inhibitor of GSK3β that can reduce tau pathology in vivo and in vitro. To identify how morin inhibits GSK3β, GSK3β protein was overexpressed and purified using affinity and ion exchange chromatography. GSK3β protein was crystallized with morin using hanging drop vapor diffusion method in the presence of 18 % (v/v) PEG 4000, 100 mM sodium citrate (pH 6.5), and 5 % (v/v) 2-propanol at 290 K. X-ray diffraction data was collected to a maximum resolution of 2.14 Å. The crystal belonged to P21, with unit cell parameters a = 67.6 Å, b = 134.4 Å, c = 100.4 Å, α = γ = 90.0°, β = 103.8°.