Abstract

Article info Vol. 7  No. 1   pp.  1 ~ 5
Title Conformational changes of JNK3 splice variants upon ATP binding
Authors Ah Young Ki, Ji Young Park and Ka Young Chung*
Institutions School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea *Correspondence: kychung2@skku.edu
Abstract c-Jun N-terminal kinases (JNKs) are members of the mitogen-activated protein kinase (MAPK) family that regulate apoptosis, inflammation, cytokine production, and metabolism. JNKs have alternative splicing at the C-terminus, resulting in long and short variants. For example, JNK3 has splice variants called JNK3α1 and JNK3α2, the short and long forms, respectively. Functional or conformational differences between the short and long splice variants have been poorly investigated. Here, we analyzed the conformational differences between JNK3α1 and JNK3α2 upon ATP-binding by using hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS showed ATP-binding affect the regions near the ATP-binding pocket in both JNK3α1 and JNK3α2 as expected. Interestingly JNK3α1 showed an additional conformational change in αL16 upon ATP-binding.