Abstract

Article info Vol. 7  No. 2   pp.  29 ~ 34
Title Advantages and operational strategies of Falcon 3EC for high resolution cryo-electron microscopy of biological macromolecules
Authors Hyeongseop Jeong1,2, Jae-Sung Woo2, Hee-seok Kweon1 and Bumhan Ryu2,*
Institutions 1Electron Microscopy Research Center, Korea Basic Science Institute, Chungcheongbukdo 28119, Republic of Korea 2Department of Life Science, Korea University, Seoul 02841, Republic of Korea *Correspondence: psryubh@korea.ac.kr
Abstract The Falcon 2 camera (Thermo Fisher Scientific Inc., USA) mounted in High Resolution Bio-TEM at Korea Basic Science Institute (KBSI) was upgraded to Falcon 3EC in August 2017 (Jeong et al., 2017). Compared with Falcon 2, Falcon 3EC has better sensitivity and higher frame rate and can be operated in electron counting mode. These changes significantly increase the data quality, thereby increasing the resolution of the 3D structure. In the benchmarking test of this machine, the structure of inhibitor-free beta-galactosidase has been solved at 2.16 Å. Here we introduce main features of the new detector, and discuss about major considerations required for its operation and data collection strategies to achieve the near atomic resolution.