Abstract

Article info Vol. 7  No. 2   pp.  35 ~ 37
Title Cloning, expression, purification, and crystallization of Xoo0878, β-ketoacyl-acyl carrier protein synthase III (FabH), from Xanthomonas oryzae pv. oryzae
Authors Ho-Phuong-Thuy Ngo1,†, Diem-Quynh Nguyen1,†, Seunghwan Kim2, Jeong-Gu Kim2, Yeh-Jin Ahn3 and Lin-Woo Kang1,*
Institutions 1Department of Biological Sciences, Konkuk University, Seoul 05029, Republic of Korea; 2Genomics Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Jeonju 03016, Republic of Korea; 3Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea *Correspondence: lkang@konkuk.ac.kr †This author contributes equally to this paper.
Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen, which causes a bacterial blight of rice. The bacterial blight is one of the most devastating diseases of rice in most of the rice growing countries and there is no effective pesticide against bacterial blight. The β-ketoacyl-acyl carrier protein synthase III (FabH) plays a key role in fatty acid synthesis (FAS) and is a promising drug target for the development of antibacterial agents. Xoo0878 gene, a fabH gene, from Xoo was cloned and its gene product Xoo0878 was expressed, purified and crystallized. Xoo0878 crystal diffracted to 2.1Å resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 57.3Å, b = 64.7Å, c = 104.2Å and α = 81.6°, β = 84.7°, γ = 74.4°. There are four monomers in the asymmetric unit, with a corresponding crystal volume per protein weight of 2.65 Å3 Da−1 and a solvent content of 53.6%. Xoo0878 structure will be useful to develop new antibacterial agents against Xoo.