Abstract

Article info 2017 5 (3)    005  (03)   pp.  96 ~ 102
Title Facility for high resolution cryo-electron microscopy of biological macromolecules at Korea Basic Science Institute
Authors Hyeongseop Jeong1, Seong-Gyu Lee2,3, Hee-Seok Kweon1 and Jaekyung Hyun1,4*
Institutions 1Electron Microscopy Research Center, Korea Basic Science Institute, Chungcheongbukdo 28119, Republic of Korea, 2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea, 4Department of Bio-analytical Science, Korea University of Science and Technology, Daejeon 34113, Republic of Korea. *Correspondence: hjk002@kbsi.re.kr
Abstract Cryo-electron microscopy (cryo-EM) single particle analysis has become a mainstream structural biology tool and therefore access to high-end transmission electron microscope (TEM) dedicated to high resolution cryo-EM is critical. At Korea Basic Science Institute (KBSI), a facility dedicated to biological electron microscopy was established in 2016, and High Resolution Bio-TEM (HR Bio-TEM, Titan Krios, Thermo Fisher Scientific Inc., USA) is now accessible to national and international researchers. This report describes major features of the HR Bio-TEM and the facility, along with overall workflow of cryo-EM image data acquisition.