Article info 2017 5 (4)    005  (04)   pp.  127 ~ 131
Title Crystallization and X-ray crystallographic analysis of the C-terminal domain of Bacillus subtilis GabR in complex with pyridoxal 5'-phosphate and γ-aminobutyric acid
Authors Seong Ah Park and Ki Seog Lee*
Institutions Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Republic of Korea *Correspondence: kslee@cup.ac.kr
Abstract Bacillus subtilis GabR (BsGabR) functions as a transcriptional activator that regulates the expression of gabTD operon involved in the γ-aminobutyric acid (GABA) catabolism. Most studies of BsGabR have been focused on the structurefunctional relationship regarding the Cterminal aminotransferase-like domain of BsGabR (BsGabR-CTD), but it still remains unclear due to lack of the structural information containing the GABA. To better understand the role of this domain, BsGabR-CTD was purified and crystallized in complex with pyridoxal 5'-phosphate and GABA. The crystal of ternary complex diffracted to a resolution of 2.0 Å and belonged to the tetragonal space group P41, with unit-cell parameters a = b = 118.497, c = 75.862 Å. Preliminary molecular replacement further confirmed the presence of one dimer in the asymmetric unit with a Matthews coefficient (VM) of 2.86 Å3 Da-1, corresponding to a solvent content of 56.9%.